Communiqué on Purity Criteria for Sweeteners Used in the Foodstuffs

5 – 8
0.5 mL saturated potasium chloride solution is added in the sample solution of 10% (weight/volume) and pH is measured. 

Isomalt, is the mixture of hydrogene mono and dissaccharides of which the basic  components are as follows:

6-O-a-D-Glucopyranosile-D-sorbitol (1.6-GPS) and 1-O-a-D-Glucopyranosile-D-mannitol dihydrate (1,1-GPM) 

6-O-a-D-Glucopyranosile-D-sorbitol C₁₂H₂₄O₁₁

1-O-a-D-Glucopyranosile-D-mannitol dihydrate C₁₂H₂₄O₁₁.2H₂O

6-O-a-D-Glucopyranosile-D-sorbitol 344.32

1-O-a-D-Glucopyranosile-D-mannitol dihydrate 380.32

Einecs:
E number
Chemical formula:
Molecular weight:
Purity:

Description:

Determination:

A- Solubility:

B- Precipitation test:

Loss of drying:
Selenium:
Lead:
Heavy metals:
Arsenic:
Cyclohexylamin:
DiCyclohexylamin:
Aniline:

Cyclohexansulphamic acid, Cyclohexylaminosulphonic acid,
202-898-1
E 952
C6H13NO3S
179.24
Cyclohexylsulphamic acid should not contain C6H13NO3S less than 98% and more than 102% in waterless base.
Sweet-sour taste, colorless, white crystal powder. About 40 fold sweeter than sucrose .

Soluble in water and ethanol
Solution of 2% is acidified with hydrochloric acid. 1 mL of about 1 molar barium chloride solution prepared with water is added and filtered if any turbidity or precipitation occurs. 1 mL of 10% sodium nitrite solution is added into the limpid solution. White precipitation is formed.

Not to exceed 1% (at 105 ºC for 1 hour)
Not to exceed 30 mg/kg based on dry matter
Not to exceed 1 mg/kg based on dry matter
Not to exceed 10 mg/kg based on lead type dry matter
Not to exceed 3 mg/kg based on dry matter
Not to exceed 10 mg/kg based on dry matter
Not to exceed 1 mg/kg based on dry matter
Not to exceed 1 mg/kg based on dry matter

Chemical name:
Einecs:
E number
Chemical formula:
Molecular weight:

Purity:

Description:

Determination:
Solubility:

Loss of drying:

Selenium:
Arsenic:
Lead:
Heavy metals:
Cyclohexylamin:
DiCyclohexylamin:
Aniline:

Sodiumcyclohexansulphamate, Sodiumcyclohexylsulfamate
205-348-9
E 952
C6H12NNaO3S and in hydrate form C6H12NNaO3S.2H2O
Waterless form; 201.22
Hydrate form; 237.22
Not to be less than 98% and more than 102% based on dry matter. Dihydrate form; not to be less than 84% based on dry matter.
White, odorless crystals or crystal powder. About 30 fold sweeter than sucrose .

Soluble in water and insoluble in ethanol.

Not to exceed 1% (at 105 ºC for 1 hour)
For dihydrate form; not to exceed 15.2% (at 105 ºC for 2 hours)
Not to exceed 30 mg/kg based on dry matter
Not to exceed 3 mg/kg based on dry matter
Not to exceed 1 mg/kg based on dry matter
Not to exceed 10 mg/kg based on lead type dry matter
Not to exceed 10 mg/kg based on dry matter
Not to exceed 1 mg/kg based on dry matter
Not to exceed 1 mg/kg based on dry matter

Chemical name:

Einecs:
E number
Chemical formula:
Molecular weight:

Purity:

Description:

Determination:
Solubility:

Loss of drying:

Selenium:
Arsenic:
Lead:
Heavy metals:
Cyclohexylamin:
DiCyclohexylamin:
Aniline:

Calcium cyclohexansulphamate, Calcium
cyclohexylsulfamate
205-349-4
E 952
C12H24CAN2O6S2.2H2O
432.57

Not to be less than 98% and more than 102% based on dry matter.

Dihydrate form; not to be less than 84% based on dry matter.
White, odorless crystals or crystal powder. About 30 fold sweeter than sucrose.

Soluble in water and insoluble in ethanol.

Not to exceed 1% (at 105 ºC for 1 hour)
For dihydrate form; not to exceed 15.2% (at 105 ºC for 2 hours)
Not to exceed 30 mg/kg based on dry matter
Not to exceed 3 mg/kg based on dry matter
Not to exceed 1 mg/kg based on dry matter
Not to exceed 10 mg/kg based on lead type dry matter
Not to exceed 10 mg/kg based on dry matter
Not to exceed 1 mg/kg based on dry matter
Not to exceed 1 mg/kg based on dry matter

Chemical name:
Einecs:
E number
Chemical formula:
Molecular weight:
Purity:

Description:

Determination:
Solubility:

Loss of drying:
Melting range:
Arsenic:
Selenium:
Lead:
Heavy metals:
Sulfated ash:
Benzoic and Salicylic acid:

o-toluensulphonamid:
p-toluensulphonamid:
Benzoic acid p-sulpho-namid:
Substances easily car-bonized:

3-oxo-2,3-dihydrobenzo(d)isotiazol-1,1-dioxide
201-321-0
E 954
C7H5NO3S
183.18
It should not contain C7H5NO3S less than 99% and more than 101% in waterless base.
Odorless or weak aromatic odor, sweet, white crystal or white crystal powder even in very high diluted solutions.  About 300-500 fold sweeter than sucrose.

Soluble in basic solutions. Soluble in water and dissolves in limited level in ethanol.

Not to exceed 1% (at 105 ºC for 2 hour)
226-230 ºC
Not to exceed 3 mg/kg based on dry matter
Not to exceed 30 mg/kg based on dry matter
Not to exceed 1 mg/kg based on dry matter
Not to exceed 10 mg/kg based on lead type dry matter
Not to exceed 0.2 mg/kg based on dry matter
3 drops of iron chloride solution of about 1 molar prepared with water, is added to the 10 mL of 1/20 solution priorly acidified with 5 drops acetic acid. Precipitation or purple color does not occur.
Not to exceed 10 mg/kg based on dry matter
Not to exceed 10 mg/kg based on dry matter
Not to exceed 25 mg/kg based on dry matter

None

Chemical name:

Einecs:
E number
Chemical formula:
Molecular weight:
Purity:

Description:

Determination:
Solubility:

Loss of drying:
Arsenic:
Selenium:
Lead:
Heavy metals:
Benzoic and Salicylic acid:

o-toluensulphonamid:
p-toluensulphonamid:
Benzoic acid p-sulpho-namid:
Substances easily car-bonized:

Sodium o-benzosulphimid,
Sodium salt of 2,3-dihydro-3-oxobenzisulphonazol
Oxobenzisulphonazol
1,2-benzizotiazolin-3-one-1
1-dioxide sodium salt dihydrate
204-886-1
E 954
C7H4NNaO3S.2H2O
241.19
It should not contain C7H4NNaO3S less than 99% and more than 101% in waterless base.
Odorless or weak aromatic odor, sweet, white crystal or white crystal powder even in very high diluted solutions.  About 300-500 fold sweeter than sucrose.

Soluble freely in water and dissolves very little in ethanol.

Not to exceed 15% (at 120 ºC for 4 hour)
Not to exceed 3 mg/kg based on dry matter
Not to exceed 30 mg/kg based on dry matter
Not to exceed 1 mg/kg based on dry matter
Not to exceed 10 mg/kg based on lead type dry matter
3 drops of iron chloride solution of about 1 molar prepared with water, is added to the 10 mL of 1/20 solution priorly acidified with 5 drops acetic acid. Precipitation or purple color does not occur.
Not to exceed 10 mg/kg based on dry matter
Not to exceed 10 mg/kg based on dry matter
Not to exceed 25 mg/kg based on dry matter

None

Chemical name:

Einecs:
E number
Chemical formula:
Molecular weight:
Purity:

Description:

Determination:
Solubility:

Loss of drying:
Arsenic:
Selenium:
Lead:
Heavy metals:
Benzoic and Salicylic acid:

o-toluensulphonamid:
p-toluensulphonamid:
Benzoic acid p-sulpho-namid:
Substances easily car-bonized:

Calcium o-benzosulphimid,
Calcium salt of 2,3-dihydro-3-oxobenzisulphonazol
Oxobenzisulphonazol
1,2-benzizotiazolin-3-one-1,1-dioxide
calcium salt hydrate (2:7)
229-349-0
E 954
C14H8CaN2O6S2.3½H2O
467.48
It should not contain C7H4NNaO3S less than 95% in waterless base.
Odorless or weak odor, sweet, white crystal or white crystal powder even in very high diluted solutions.  About 300-500 fold sweeter than sucrose.

Soluble freely in water and soluble in ethanol.

Not to exceed 13.5% (at 120 ºC for 4 hour)
Not to exceed 3 mg/kg based on dry matter
Not to exceed 30 mg/kg based on dry matter
Not to exceed 1 mg/kg based on dry matter
Not to exceed 10 mg/kg based on lead type dry matter
3 drops of iron chloride solution of about 1 molar prepared with water, is added to the 10 mL of 1/20 solution priorly acidified with 5 drops acetic acid. Precipitation or purple color does not occur.
Not to exceed 10 mg/kg based on dry matter
Not to exceed 10 mg/kg based on dry matter
Not to exceed 25 mg/kg based on dry matter

None

Chemical name:

E number
Chemical formula:
Molecular weight:
Purity:

Description:

Determination:
Solubility:

Loss of drying:
Arsenic:
Selenium:
Lead:
Heavy metals:
Benzoic and Salicylic acid:

o-toluensulphonamid:
p-toluensulphonamid:
Benzoic acid p-sulpho-namid:
Substances easily car-bonized:

Potassium o-benzosulphimid,
Potassium salt of 2,3-dihydro-3-oxobenzisosulphonazol
Potassium salt of 1,2-benzisotiazolin-3-one-1,1-dioxide mono hydrate
E 954
C7H4KNO3S.H2O
239.77
It should not contain C7H4KNO3S less than 99% and more than 101% in waterless base.
Odorless or weak odor, sweet, white crystal or white crystal powder even in very high diluted solutions.  About 300-500 fold sweeter than sucrose.

Soluble freely in water and dissolves very little in ethanol.

Not to exceed 8 % (at 120 ºC for 4 hour)
Not to exceed 3 mg/kg based on dry matter
Not to exceed 30 mg/kg based on dry matter
Not to exceed 1 mg/kg based on dry matter
Not to exceed 10 mg/kg based on lead type dry matter
3 drops of iron chloride solution of about 1 molar prepared with water, is added to the 10 mL of 1/20 solution priorly acidified with 5 drops acetic acid. Precipitation or purple color does not occur.
Not to exceed 10 mg/kg based on dry matter
Not to exceed 10 mg/kg based on dry matter
Not to exceed 25 mg/kg based on dry matter

None

Chemical name:

Einecs:
E number
Chemical formula:
Molecular weight:

Purity:

Description:

Determination:
Solubility:

Loss of drying:
Carbohydrates:
Sulphated ash:
Aluminium:
Arsenic:
Lead:
Microbiological criteria:

Taumatin, is produced by watery extraction (pH 2.5-4.0) of seed skins of the fruits of natural sorts of Thaumatococcus danielli (Benth) and basically contains taumatin I and taumatin II proteins together with very minute amount of plant components composed of source material.
258-822-2
E 957
207 polypeptide of aminoacids.
Taumatin I: 222.09
Taumatin II: 222.93
It should not contain nitrogen less than 16 % equivalent (Nx5.8) to protein not less than 94% in dry matter base.
Odorless, cream colored, densely sweet powder. About 2000-3000 fold sweeter than sucrose.

Soluble  in water very much, insoluble in acetone.

Not to exceed 9 % (at 105 ºC up to a constant weight)
Not to exceed 3 % based on dry matter
Not to exceed 2 % based on dry matter
Not to exceed 100 mg/kg based on dry matter
Not to exceed 3 mg/kg based on dry matter
Should be 3 mg/kg based on dry matter
Total aerobic microbiologic count: 1000/g
                                    E. coli: should not exist in 1 g.

Chemical name:

E number
Chemical formula:
Molecular weight:
Purity:
Description:

Determination:

A- Solubility:

B- Maximum ultra-violet absorption:

C. Neu test:

Loss of drying:
Sulphated ash:
Arsenic:
Lead:
Heavy metals:

2-O-?-L-ramnopyranosyl-4’-ß-D-glucopyranosyl hespere-tin dihydrocalcon produced by catalytic hydrogenation of neohesperidyn.
E 959
C28H36O15
612.6
Not to be less than 96% based on dry matter.
Dark white colored, odorless, crystal powder having characteristic dense taste. About 1000-1800 fold sweeter than sucrose.

Freely soluble in hot water, very little soluble in cold water. Insoluble in ether and benzene.
282-283 nm for solution containing 2 mg, in 100 mL methanol.
10 mg neohesperidyn dissolves in 1 mL methanol. 1 mL of 1% 2-aminoethyl diphenyl borate methanolyc solution is added. Light yellow color is observed.
Not to exceed 11 % (at 105 ºC for 3 hours)
Not to exceed 0.2 % based on dry substance
Not to exceed 3 mg/kg based on dry substance
Should be 2 mg/kg based on dry substance
Not to exceed 10 mg/kg based on lead type dry substance