T.C.
TARIM ve KÖYİŞLERİ BAKANLIĞI
Koruma ve Kontrol Genel Müdürlüğü
PURIFICATION OF LIVE BIVALVE MOLLUSCS
SHBHM Circular 2001-02
A. OBJECTIVE: Bivalve molluscs that are collected or farmed in our country are subjected to certain analyses by the Ministry of Agriculture and Rural Affairs before they are presented for human consumption. This circular has been prepared in order to specify the operations which are required to decontaminate from microbiological contaminants and to make suitable for human consumption the live bivalve molluscs, which can not be directly supplied for human consumption due to their high level of microbiological load as indicated by the results of the microbiological analyses performed in this respect, and to get such operations implemented as a standard.
B. DEFINITIONS: For the purposes of this Circular, the important terms used herein shall have the following meanings hereafter:
1. Bivalve Molluscs: Two symmetrically shelled aquatic products which feed on nutrients that are available in the aquatic environment by filtering water and the body of which are enclosed between two hinged shells and which are also found in fresh water environment. (venus veroccuso, Mytilus galloprovincialis, modiolus spp., Oyster, carpet-shell, Venus verrucosa)
2. Production Area: Areas such as sea fields, estuaries or artificial lagoons which harbour clusters of bivalve molluscs, and areas where bivalve molluscs are farmed.
3. Relaying Area: Sea and lagoon areas granted and depicted by the Ministry and clearly demarked for natural purification of bivalve molluscs.
4. Purification Centre: Centres where ponds or tanks exist to accommodate live bivalve molluscs for an adequate duration of time to decontaminate them from microbiological contaminants in order to make them suitable for human consumption, where they naturally feed on clean sea water or on sea water treated by adequate operations.
5. Centre of Dispatch (Packaging Enterprise): Premises established at or off the shore for receiving, improvement, washing, cleaning, classification and packaging of bivalve molluscs that are suitable for human consumption.
6. Purification Water: Sea water or brackish water that is free of microbiological contamination that naturally occurs in the environment or that occurs due to wastes, and that does not adversely effect the health conditions of bivalve molluscs or that does not contain toxic and undesired material at quantities to deteriorate the quality of the bivalve molluscs, and that satisfy the criteria given in APPENDIX-1 (as indicated in Directive 79/923/EEC).
In addition, water which is obtained by adding basic chemical compounds into water bearing the qualities indicated in Directive 80/778/EEC may also be used as purification water.
C) MINIMUM REQUIREMENTS FOR PURIFICATION CENTRES
1. Requirements for Buildings and Equipment
1.1 Centres for purifying live bivalve molluscs:
1.1.1 They must be installed particularly at or near the shore with a view to making use of sea water. However, they may also be installed at other sites if the purification water is obtained by adding chemical compounds into a water which is in compliance with Directive 80/778/EEC.
1.1.2 Necessary measures and precautions must be taken against every kind of contamination and pollution which may arise from environment due to the location of site.
1.1.3 It must posses covered hygienic facilities or buildings, ponds and tanks which are adequately protected from and designed to prevent contamination of bivalve molluscs by every kind of dirt, waste water, smoke, waste, mice or other animals.
1.1.4 It must posses a working area sufficient to perform all activities adequately.
1.1.5 Administrative units and locker rooms, bathroom and toilets, and social units such as cafeteria shall not have any direct contact with the areas where purification ponds and tanks exist.
1.1.6 It must have a floor and a drainage system which is easy to clean and able to discharge waste water.
1.1.7 Walls must be easy to clean, impermeable, smooth and firm.
1.1.8 Ceiling must have a design that prevents evaporation and dripping.
1.1.9 All doors and windows in the work area must be designed to prevent passage of dust, pests and mites.
1.1.10 It must have adequate, natural or artificial lighting.
1.2 There must be appropriate locker rooms, lockers, shower cabinets, toilets and lavatories in sufficient number for the personnel.
1.3 There must be sufficient and appropriate equipment and space to wash tools, containers and equipment. Tools and equipment and surfaces which come into contact with live bivalve molluscs must be made of a material which can be repeatedly washed and easily cleaned but resistive against wear.
2. Technical Specifications for Ponds and Tanks
2.1 Ponds and tanks must be installed at a covered area so that they will not be effected by sunlight or external effects.
2.2 Materials used to construct the ponds and tanks must not leave chemical residues and not have any toxic effect on the product.
2.3 Bases and walls of the purification tanks and ponds, and all kinds of water holding containers shall have smooth, hard and impermeable surfaces and must be easily cleaned by rubbing by a brush or by using pressurized water.
2.4 Bases of the purification tanks and ponds must have an appropriate slope (min 1/100).
2.5 Heating and cooling systems must be installed at the sea water inlet in order to keep the sea water temperature constant.
2.6 Ponds and tanks must be equipped with proper discharge system. This discharge system must be able to discharge the same quantity water as the inlet water.
2.7 Discharge pipe of the pond or tank must be 25 mm above the pond or tank base.
2.8 There shall be a second drainage pipe attached to the pond or tank in order to remove the waste of bivalve molluscs and sediments collected at the base from environment.
2.9 A flow-meter shall be installed at the inlet of the ponds and tanks.
2.10 Sufficient flow of purification water must be ensured for purification tanks and ponds, considering the type and quantity of the live bivalve molluscs and duration.
2.11 There must be a steady flow of purification water into the pond or tank. The flow rate of purification water must be sufficient to provide water change within an hour in the pond or tank.
2.12 There must be a UV source of minimum 30 watts for every 2.2 m3 of sea water flowing into the tank or pond in order to sterilize it and this source must be safely secured in place. Additionally, there shall be a filter system to retain the solid particles contained in sea water. Operation of the system must be controlled.
2.13 The ratio of the bivalve mollusc stock quantity to the purification water within every pond or tank shall be minimum 1/3. (For example: 2600-9600 lt of clean sea water must be supplied for every tank or pond with a capacity of 750 kg or 1500 kg mussel. Water flow rate in a system with the mentioned capacity must be 12.5 m3/hour.)
2.14 Boxes or crates which hold bivalve molluscs must be placed at least 5 cm above the base of the pond or tank.
2.15 Maximum two rows of oysters, cockles or clams shall be laid in each box or cage. The thickness of the mussels laid in every box or cage must be maximum 8 cm.
2.16 Water level in the pond or tank must be minimum 8 cm above the surface of the products.
2.17 Boxes or crates which contain live bivalve molluscs and which are placed into purification tanks or ponds must have a structure to let the sea water flow through them.
2.18 Special systems which do not bear the above mentioned characteristics can be accepted if deemed appropriate following their inspection by the Ministry.
3. General Hygiene Conditions
Sufficient and appropriate sanitary and hygiene rules shall be implemented at purification centres with respect to personnel, work places, equipment and working conditions.
3.1 Personnel who handle live bivalve molluscs and process them by hand must particularly wear clean work clothes and where appropriate suitable gloves. The protective dresses and overalls that are used during work shall not be used outside the work area at social premises.
3.2 There shall be disinfection systems at the entrance of work places consisting of lavatories which are not manually operated, liquid soap dispenser, paper towel and waste basket, and there shall be feet disinfection pools.
3.3 Periodical health inspection of employee shall be carried out. Personnel must refrain from personal habits like spitting which might infect the live bivalve molluscs. Any person who poses a disease risk for the product shall not be allowed to contact with the product until he is healed.
3.4 A general Pest Fight Plan shall be prepared and executed for purification centres. All kinds of rodents, pests and other vermin at site shall be eliminated and all necessary measures shall be taken within the framework of this pest fight plan. Domestic animals shall be prevented to enter the site of premises.
3.5 A general Sanitation Plan shall be prepared and executed for purification centres. Ponds and tanks, tools and equipment that are used in purification operations of live bivalve molluscs must be maintained under clean conditions.
3.6 Tools and equipment existing at purification centres shall not be used for purposes other than operations involving live bivalve molluscs.
3.7 Refuse products must be stored at a separate place under hygienic conditions and, if possible, within closed containers that are properly marked for that purpose. Waste material must be removed from the purification centre at regular intervals.
3.8 Final products that have completed the purification process must be kept or stored at a closed place and must be kept away the areas where aquatic products other than live bivalve molluscs, like crustacean (shrimps, crayfish, etc.) are processed.
4. Properties of Water Used at Purification Centres
4.1 When sea water is being used in the tanks or ponds at purification centres, the purification water must conform to the criteria specified in APPENDIX-1.
4.2 Sea water must be driven from points which are away from discharge regions or bad quality surface waters or away from points of suction of bottom mud or sand.
4.3 Sea water must have a clear appearance and must not be turbid.
4.4 Salinity of the sea water used must be equivalent to the salinity of the production area where the products were collected, or else, it must be rendered as equivalent.
4.5 Sea water must be passed through a UV system prior to its entrance into the tank or pond in order to eliminate its microbiological contamination.
4.6 Equal distribution of dissolved oxygen must be ensured throughout the purification water during the purification process.
4.7 The potable water that is used to obtain sea water by using main chemical compounds must bear the properties specified in Directive 80/778/EEC.
4.8 Sedimentation pools should exist if purification water is being obtained by artificial ways and permanently used in the system. These pools must be designed as to discharge the accumulating waste.
4.9 Effluent water from purification process shall be discharged at a region away from the sea water suction point.
5. Other Conditions
Purification centres shall satisfy the following requirements in addition to those specified in Articles 1, 2, 3 and 4.
5.1 Live bivalve molluscs must be washed for an adequate duration prior to purification by pressurized sea water or potable water free of mud. Preliminary washing may be performed within purification tanks or ponds before the start of the purification process. Drainage pipes must be kept open in such a case.
5.2 The distance between the sea water inlet point and the waste water outlet point must be long enough to prevent contamination. If the sea water requires treatment it must be a procedure that is approved by the Ministry as appropriate.
5.3 The quantity of the live bivalve molluscs to be purified must not exceed the capacity of the purification centre.
5.4 All live bivalve molluscs which are to be laid into the same purification tank or pond must be of the same species. This species might have been taken from the same production area, or from different production areas bearing the same microbiological load. If the products are from different production areas with similar microbiological load, tracing them in related documents must be ensured.
5.5 No aquatic products such as shelled aquatic products, fish or other sea species shall exist within the purification tanks or ponds where live bivalve molluscs are processed for purification.
5.6 Following the completion of purification, shells of live bivalve molluscs must be washed by hose-water by using potable or sea water. This operation may be performed in the purification tank if required. But the washing water may not be re-used.
5.7 Purified live bivalve molluscs must be packed and marketed following purification. They shall not be left in sea water or any other water environment.
5.8 Purification centres must have their own laboratories. Purification centres without a laboratory shall use the laboratories of the Ministry.
D) CLASSIFICATION OF PRODUCTION AREAS
1. Production areas of live bivalve molluscs are grouped in three classes, A, B and C, according to their microbiological load.
1.1 Class A production areas are those whose borders are determined by the Ministry, implementing a monitoring programme and which present the following results at microbiological analysis:
- contains less than 300 EMS Faecal coliform or less than 230 EMS E. coli in 100 gr flesh and intra-valvular fluid,
- does not contain salmonella in 25 gr. flesh,
- does not contain Vibrio parahaemolyticus,
- does not contain DSP toxicologically; with the quantity of PSP being less than 80mg/100gr; and the quantity of ASP being less than 20 μg/gr,
- Maximum radionuclide Cs-137 quantity does not exceed 600 Bq/kg,
- chemically, the results of heavy metal analysis does not exceed 20.0 mg/lt for copper, 50.0 mg/lt for zinc, 0.5 mg/lt for mercury, 0.1 mg/lt for cadmium, 1.0 mg/lt for lead, 1.0 mg/lt for arsenic.
1.2 Class B production areas are those whose borders are determined by the Ministry, implementing a monitoring programme and which present the following results at microbiological analysis:
- contains 300-6000 EMS Faecal coliform or 230-4600 EMS E. coli in 100 gr flesh and intra-valvular fluid,
- does not contain salmonella in 25 gr. flesh,
- does not contain Vibrio parahaemolyticus,
- does not contain DSP toxicologically; with the quantity of PSP being less than 80mg/100gr; and the quantity of ASP being less than 20 μg/gr,
- Maximum radionuclide Cs-137 quantity does not exceed 600 Bq/kg,
- chemically, the results of heavy metal analysis does not exceed 20.0 mg/lt for copper, 50.0 mg/lt for zinc, 0.5 mg/lt for mercury, 0.1 mg/lt for cadmium, 1.0 mg/lt for lead and 1.0 mg/lt for arsenic.
1.3 Class C production areas are those whose borders are determined by the Ministry, implementing a monitoring programme and which present the following results at microbiological analysis:
- contains 6000-60 000 EMS Faecal coliform in 100 gr flesh and intra-valvular fluid,
- does not contain salmonella in 25 gr. flesh,
- does not contain Vibrio parahaemolyticus,
- does not contain DSP toxicologically; with the quantity of PSP being less than 80mg/100gr; and the quantity of ASP being less than 20 μg/gr,
- Maximum radionuclide Cs-137 quantity does not exceed 600 Bq/kg,
- chemically, the results of heavy metal analysis does not exceed 20.0 mg/lt for copper, 50.0 mg/lt for zinc, 0.5 mg/lt for mercury, 0.1 mg/lt for cadmium, 1.0 mg/lt for lead and 1.0 mg/lt for arsenic.
2- These production areas are determined by the Ministry and announced to the concerned.
3- Bivalve molluscs obtained from Class A production areas may be directly presented to the domestic market or to foreign markets.
4- Bivalve molluscs obtained from production areas that contain 300 - 6000 EMS Faecal Coliform or 230 - 4600 EMS E. coli although such areas are Class A areas may be presented to the domestic market or foreign market after they are purified.
5- If it is desired to present the bivalve molluscs obtained from Class B production areas to the domestic market or foreign market as live animals, such bivalve molluscs should be purified. Purification centres may only receive products from production areas of Class B and those specified in Article 4.
6- It is mandatory that the bivalve molluscs obtained from Class C production areas are kept in the relaying area and then subjected to purification. However, since there are no identified relaying areas in Turkey, no products should be received directly from Class C production areas for exportation, processing, purification or relaying.
E) APPROVAL OF PURIFICATION CENTRES
Real or judicial persons who found purification centres apply to the Provincial Directorates of the Ministry, together with the information, documents and projects demonstrating the technical and hygienic specifications of the purification centres. The personnel of the Provincial Directorates of the Ministry conduct an inspection in the purification centre, taking into account the minimum requirements in this circular and the results are determined by a minutes.
If it is concluded that the status of the purification centre is appropriate and compliant, the minutes recorded is communicated to the Ministry, together with the opinion of the Provincial Directorate. If incomplete points have been determined, pertaining information is communicated to the concerned real or judicial person. The application should not be sent to the Ministry without first correcting the determined deficiencies.
The Ministry carries out inspections on site concerning the compliance of the purification centres with this circular. The following controls are conducted during such inspection:
1. Firstly, the system is filled with only purification water and it is checked whether water circulation is ensured. In such controls, whether there are leakages in connections and walls of the tanks or pools and whether the part that provides water into the spray pool at the inlet of the pool or tank are also checked. Then, the tank or pool is emptied. Whether the tank or the pool is completely emptied after discharge is checked.
2. The purification process should be based on lot. Firstly, the bivalve molluscs to be purified are placed into the tank. Then, the tanks or pools are filled with water and water flow is monitored. After a time approved for the system, the product is completely taken out and is subjected to the required analyses. After the product is placed in the tank or pool, no product should be added or removed.
3. Bivalve molluscs obtained from Class A production areas may be directly presented to the domestic market or to foreign markets.
4. In case there is a closed circuit purification system in the establishment, studies to determine the exchange time of the purification water must be carried out.
5. In facilities here there is no heating and cooling system, approval should be periodical.
The Ministry issues an approval number to the purification centres that are deemed as compliant as a result of all these inspections and such number is communicated to the concerned. If exportation is being made from the purification centres is being made, such approval numbers are communicated to the relevant units of the European Union. After being published in the Official Gazette of the EU, such approval numbers come into force.
The purification centres for which an approval number has been issued should continue their operations in parallel with the procedures specified and approved by the Ministry. The approval numbers of the approved purification centres may be cancelled or suspended temporarily by the Ministry depending on the results of inspection.
F) PURIFICATION PROCESSES AND ANALYSES
1. Reception of Bivalve Molluscs
1- The purification centres receive only Class B bivalve molluscs and only from the production areas specified in Article D.4. For bivalve molluscs received from Class A and B production areas (in compliance with Article D.4) to be purified, an origin of certificate, a model of which is given in ANNEX-2, is issued by the Provincial or District Directorates. This document must bear a stamp, stating “This product has been collected in a Class A or B production area (in compliance with Article D.4), and SHALL BE PURIFIED). This stamp shall be red in colour.
2- Purification centres may only receive the bivalve mollusc batches accompanied with its certificate of origin. Products which do not have a certificate of origin should not be subjected to any process in the purification centre.
3- Reception, handling and separation processes of the product should be duly cared for. If the death rate is high and if the valves are open in the products coming to the centre, these products should be rejected. The product should readily be started with the purification chain after it has been received. However, bivalve molluscs taken from the production area may be kept waiting in the sea area in the region where the purification centre is located.
4- The duration of validity of the certificate of origin of the bivalve molluscs is 45 days, starting with the date of issuance. Live bivalve molluscs should be subjected to purification within maximum 10 days of its date of issuance. In case such duration is exceeded, the product should be subjected to microbiological and toxicological analyses, and if the results of such analyses exceed the following conditions, should be re-purified:
- contains 300-6000 EMS Faecal coliform or 230-4600 EMS E. coli in 100 gr flesh and intra-valvular fluid,
- does not contain salmonella in 25 gr. flesh,
- does not contain Vibrio parahaemolyticus,
- does not contain DSP toxicologically; with the quantity of PSP being less than 80mg/100gr; and the quantity of ASP being less than 20 μg/gr, The bivalve molluscs exceeding these limits shall not be subjected to purification in the purification centre.
2. Purification Processes
1) After bivalve molluscs are brought to the purification centre and after they are washed, removing sand, clay, weeds and mud, they are placed in cases or boxes and put into purification tanks or pools. When placing, the position and direction of the cases or boxes should be given care. The shells of the bivalve molluscs placed in tanks or pools should be slightly movable (opening and closing). It should be observed that the shells become more movable a few hours after the start of the purification, depending on the species. The day following purification, bivalve molluscs must absolutely be active.
2) During purification the tank or pool bottom should be visible. Otherwise, the system should e emptied and the problem should be investigated. Foam formation may be observed during purification, depending upon the species. It should be cared that such formation is not excessive. When water is being fed into the tank or pool, it should be checked whether the UV system is in operation. If not, it should be started.
3) Live bivalve molluscs are purified continuously for a sufficient time to decrease the microbiological load. This duration starts when the live bivalve molluscs in the purification tanks or pools are covered sufficiently with water and continues until they are taken out. However, an excessively long purification process leads to the death or weakening of the product. In determining the duration of purification, the time which ensures the intended purification should be taken into account. If the system is stopped or if the amount of water is decreased, there may be product loss. If weakening, excessive opening or death is observed in the products, such products may not be offered for consumption as human food. The purified bivalve molluscs should comply with the tolerance values given in ANNE-3.
4) If a closed circuit purification system exists in the purification centre, maximum utilisation time of the purification water, parameter measurement and compliance of the water quality should be monitored by responsible personnel. If the bivalve mollusc in the system appears inactive or if a doubt arises concerning the water quality, new purification water should be added or exchanged. If the bivalve mollusc becomes inactive, the purification process should be repeated.
5) The tanks or pools in which the cases or boxes are placed should bear a plate, a model of which is given in ANNEX-4, stating the information in the certificate of origin. This plate should be filled by the technical responsible person of the establishment.
6) New product cannot be placed in the purification tank or pool without completely taking out the products placed formerly. Before bivalve molluscs are taken out, the water in the tank or pool should be emptied. During this process, the UV lamp and the pump may be shut off. There may be some water at the base, but such water should never be at the product level. After emptying, the tank or pool should be washed. Final product must be alive and the processes should be carried out caring not to cause a shock or damage on the product.
7) Following the purification process, the tank or pool should be emptied, washed with clean water and sand and wastes at the bottom must be cleaned away. Mud or deposits in the discharge pipe should be cleaned regularly to avoid plugging. For cleaning, hypochlorite or cleaning materials may be used. The cleaning may be carried out by adding hypochlorite after the tank or pool is filled. The tank or pool should be washed thoroughly folloing cleaning to avoid any chlorine residues.
8) After the purification process is completed, the purification document in ANNEX-5, for the bivalve molluscs is issued by the technical responsible personnel of the purification centre. This document shall be printed as a notebook with successive serial numbers. The names and signature statements of the personnel who will issue this document should be communicated to the Provincial Directorates by the purification centres, together with statement of undertaking which states that the document should be completed fully and that the information contained shall be true.
3. Analyses
1) The purification centres should have knowledge on the results of the microbiological analyses conducted on the live bivalve molluscs coming to the establishment. The results of the analyses concerning the production areas should be received together with the certificate of origin.
2) The duration of purification required to reduce the microbiological load of the bivalve molluscs to be subjected to purification is communicated to the purification centre by the Ministry. The pertaining analysis results are kept in the purification centre.
3) For controlling purposes, the inspectors of the Ministry conduct microbiological analyses on bivalve molluscs, sampling once monthly. The samples taken should be of sufficient quantity for the analyses to be performed. For such analyses, at least 1 kg of live bivalve molluscs, based on species. Samples should not be dead or opened. The sample taken should be placed in clean nylon bags, be kept cold at 4°C during transportation and handed to the laboratory within 24 hours. Samples of bivalve molluscs should never be frozen.
4) The analyses of the water to be used in purification should be carried out in periods given in ANNEX-1. Daily measurements and samples for analyses should be taken by the technical responsible personnel of the purification centre. The samples taken monthly for microbiological analyses and taken semi-annually for heavy metal analyses shall be taken by the inspectors of the Ministry, with proper methods and in appropriate quantities. In case one of the parameters is positive, the purification process is stopped. Such products cannot be exported or placed on the market. However, they may be marketed after re-purification if the parameters concerning water are corrected. When analyses performed successively are found to be negative twice, the purification process may be re-started.
G) LABEL
The purified live bivalve molluscs shall bear a label containing the minimum information given in ANNEX-6 to indicate that all bivalve molluscs have been purified.
H) RECORDS
The purification centres keep available at all times:
1) the reports showing the results of the physical, chemical and microbiological analyses of the purification water fed into purification tanks or pools;
2) the reports showing the results of the microbiological analyses conducted on non-purified live bivalve molluscs;
3) the reports showing the results of the microbiological analyses conducted monthly on purified live bivalve molluscs for self-control;
4) the certificates of origin of the live bivalve molluscs coming into the purification centre;
5) the purification certificates issued by the technical responsible personnel of the purification centre for purified live bivalve molluscs;
6) the reports showing the results of the analyses related to the studies on purification durations depending on product species and pollution load; and
7) if the purification centre carries out exportation business, the health certificates of the exported live bivalve molluscs or the documents related to the place where the product from purification is sent,
in the centre, in order to be presented to the inspectors in the inspections conducted by the Ministry
8) The purification centres keep a record book, an example of which is given in ANNE-7. The date of arrival of the live bivalve molluscs to the purification centre and the starting and completion times of purification shall be recorded separately for every lot. In addition to these recordings, the salinity and temperature of water and the duration of UV source application must be recorded. In the records, there must be an explanations column where the events occurring during purification should be written (such as pump breakdown and the actions taken). The record book should be kept dry and each page should be numbered.
I) EXPORTATION (MARKETING)
The preliminary health certificate or the health certificate shall be issued by the veterinarians, whose names are listed in the inspectors list approved by Ministry and who inspect the establishment. When a preliminary health certificate or a health certificate is being issued by the veterinarians, the followings should be inspected:
- the presence of the certificates of origin and the purification document of the purified live bivalve molluscs;
- whether the certificates of origin are taken from Class B production areas;
- by controlling the records, whether the amount of exported lot and the purified product and product species are the same;
- whether bivalve molluscs have been purified for an appropriate duration;
- if sea water is being used, the results of the microbiological analyses of the sea water and whether such analyses have been carried out at the specified intervals;
- for control, the results of the analyses carried out on purified products and whether such analyses have been carried out at appropriate intervals.
ANNEX-1
QUALITY CRITERIA FOR SEA WATER TO BE USED IN PURIFICATION CENTRES
| PARAMETERS TO BE ANALYSED | APPROPRIATE LIMITS | REFERENCE ANALYSIS METHOD | SAMPLING PERIODS AND MEASUREMENT | |
| 1 | Ph | 7-9 | - Electrometric measurement (measurement conducted on site during sampling) | Daily |
| 2 | Temperature °C | The temperature of sea water taken from natural medium and used to fill purification tanks or pools should not change more than 2°C. | - Measurement by a thermometer | Daily |
| 3 | Salinity (%0 ) | - < 40 ( preferably between 12-38 %) | Conductivity metre Salinometre | Daily |
| 4 | Dissolved Oxygen | Should be below 6 mg/lt | - Winkler method - Electrochemical method (O2 metre) | Daily |
| 5 | Petroleum hydrocarbons | There should not exist a visible layer on the surface where sea water is taken | Visual inspection | Daily |
| 6 | Heavy metals Silver (Ag) mg/l Arsenic (As) mg/l Cadmium (Cd) mg/l Chromium (Cr) mg/l Copper (Cu) mg/l Mercury (Hg) mg/l Nickel (Ni) mg/l Lead (Pb) mg/l Zinc (Zn) mg/l |
0.003 0.1 0.01 0.1 0.01 0.004 0.1 0.1 0.1 |
Measurement at appropriate concentration and/or extraction i,n Atomic Absorption Spectrophotometer., | Once in six months |
| 7 | Faecal Coliform | Should not exist | Multi tube method (3 solutions, 5 experiment tubes) | Once in six months |
| 8 | E. Coli | Should not exist | Multi tube method (3 solutions, 5 experiment tubes) | Once in six months |
| 9 | Toatal Coliform | Should not exist | Multi tube method (3 solutions, 5 experiment tubes) | Once in six months |
ANNEX-2
CERTIFICATE OF ORIGIN FOR BIVALVE MOLLUSCS
| Of the Certificate of Origin | Date …/…/….. |
No …./…. |
Issuing Institution |
| Of the product | Species : | Amount (Kg) | |
| Purpose of evaluation (1) | |||
| Name of the production region: | Code : | ||
| Name of the production area: | Number: | ||
| Of the real or judicial person producing the product | Name, Surname/Title | Fishery products fishing permit No : | |
| Address : | Signature : | ||
| Of the establishment where the product is to be dispatched | Name: | ||
| Approval No : | |||
| Location : | |||
| Amount of product dispatched to the establishment (Kg) | |||
| Of the real or judicial person transporting the product | Name, Surname/Title | ||
| Type and licence number of the transportation vehicle: Transportation capacity (Ton) : |
|||
(1) Live or Processed
Of the Authorised Person Issuing the Document
Name, Surname :
Title :
Signature-Seal :
ANNEX-3
PARAMETERS TO BE SOUGHT IN LIVE BIVALVE MOLLUSCS TO BE OFFERED DIRECTLY FOR HUMAN CONSUMPTION
| PARAMETERS | TOLERANCE VALUES | |
| Organoleptic (X1) | Appearance | Fresh and live, shells free of dirt, appropriate response to impacts and normal level of intravalvular fluid |
| Microbiologic (X2) | Faecal Coli | 300 EMS/100 gr. |
| E. Coli | 230 EMS/100 gr. | |
| Salmonella | Should not exist in 25 gr. | |
| V. parahaemolyticus | Should not exist | |
| Toxicological (X3) | DSP | Should not exist |
| PSP | 80 µg./100 gr. | |
| ASP | 20 µg/gr. | |
| Chemical (X3) - Heavy Metals - Radioactive Load |
Mercury | 0.5 mg/kg. |
| Cadmium | 0.1 mg/kg. | |
| Lead | 1.0 mg/kg. | |
| Copper | 20.0 mg/kg. | |
| Zinc | 50.0 mg/kg. | |
| Arsenic | 1.0 mg/kg. | |
| Radionuclides | Cs–137 : 600 Bq/ kg | |
X1 : Conducted for every product lot from purification.
X2 : Conducted for every product species monthly for control.
X3 : Conducted at the production sites.
ANNEX-4
PLATE TO BE PLACED ON TANKS OR POOLS
| Tank or Pool | No | |
| Certificate of Origin | Date .../.../20.. |
No ...../...... |
| Of the Production Region(s) | Name | Code |
| Of the Production Area(s) | Name | No |
| Of the Bivalve Mollusc | Species | Amount (kg) |
| Date of arrival of the bivalve molluscs to the purification centre | ||
| Total amount taken for purification (kg) | ||
| DURING PURIFICATION | At the start of the process | After 2-3 hours following start | Mid-time of the purification process | At the end of the process | ||||
| Date | ......./......../....... |
......./......../....... | ||||||
| Duration | .......... hours | .......... hours | ||||||
| Is the water level appropriate? | Yes |
No |
Yes |
No |
Yes |
No |
Yes |
No |
| Is the water flow appropriate? | Yes |
No |
Yes |
No |
Yes |
No |
Yes |
No |
| Is the UV lamp in operation? | Yes |
No |
Yes |
No |
Yes |
No |
Yes |
No |
| Water temperature | °C | °C | °C | °C | ||||
| Is the appearance and odour of water appropriate? | Yes |
No |
Yes |
No |
Yes |
No |
Yes |
No |
| Are the bivalve molluscs active? | Yes |
No |
Yes |
No |
Yes |
No |
Yes |
No |
| Name, Surname and Signature of the responsible person conducting the operation | ||||||||
| AFTER PURIFICATION | Has the sea water been discharged? | Yes |
No |
| Has the Tank or pool been cleaned? | Yes |
No |
|
| Total operation time of the UV | .............. hours | ||
| Explanations ( information on adverse events, their causes and the actions taken shall be given) |
Of the Authorised Personnel of the Purification Centre Issuing the Document
Name :
Surname :
Title :
Signature :
ANNEX-5
PURIFICATION DOCUMENT FOR LIVE BIVALVE MOLLUSCS
Serial No:
| Of the purification document | Date …/…/….. |
No …./…. |
|
| Of the product | Species : | Amount purified (Kg) | |
| Production area class: | Of the purified product’s certificate of origin; Date No ...../....../ .... ....../....... |
||
| Name of the production region: | Code : | ||
| Name of the production area: | No: | ||
| Of the product | Date of arrival in the establishment
...../......./...... |
Time . ........... |
|
| Of purification | Date of start ......../......./....... | Time ........... | |
| Date of end ......./......./........ | Time ........... | ||
| Of sea water | Amount of Dissolved Oxygen | Salinity | |
| Temperature | Other characteristics | ||
| Of the centre where purification was conducted | Name: | ||
| Approval No : | |||
| Address : | |||
| Valuation type of the purified product | |||
| Exportation ‘
Processing and valuation ‘ Domestic market ‘ |
Name: | ||
| Approval No : | |||
| Address : | |||
Of the Authorised Personnel of the
Purification Centre Issuing the Document
Name, Surname :
Title :
Signature:
ANNEX-6
LABEL MODEL FOR PURIFIED BIVALVE MOLLUSCS
| Of the species of the bivalve mollusc | Commercial name :
Scientific name : |
| Of the Purification Centre | |
| Name | |
| Approval No | |
| Address | |
| Packaging | |
| Date of packaging | |
| Material of packaging | |
| Shelf life | These bivalve molluscs must be alive when sold. |
| PURIFIED | |
Note : A different label can be designed provided that it contains the above information.